To undertake experimental data statistical analysis and processing business - Database & Sql Blog Articles

The company has technical personnel with public health research background, and now undertakes statistical analysis and processing of experimental data for customers.
OCT embedding agent (SAKURA product, MADE IN USA) embedding process:

1. Remove the tissue block placed in the sucrose solution, put in a mixture of 20g L-1 sucrose and OCT embedding agent in a 1:1 volume ratio, soak for 2h at room temperature.

2. Move into OCT embedding agent for 4h at room temperature, replace with new OCT embedding agent, soak for 6h at room temperature.

3. Place the specimen on the pallet, slice it with a cryostat slicer (generally -22 ° C), slice 10 μm, attach it to the pre-treated slide, and store in a -20 ° C refrigerator.

OCT embedding agent

English full name: opti-mum cutting temperature compound OCT

The frozen section embedding agent is a water-soluble mixture of polyethylene glycol and polyvinyl alcohol. It has been widely used in immunohistochemistry laboratories. Its purpose is to support tissues during frozen sectioning to increase tissue continuity and reduce Wrinkles and cracks.

Since the OCT mixture is water-soluble, it is soluble in water during the bleaching, so that background dyeing is not increased in subsequent dyeing.

The tissue was pre-infiltrated with an opti-mum cutting temperature compound and then subjected to cryostat sectioning to improve the quality of the sections.

For more details, please call us! !