Recently, Hu Student of Gansu University of Traditional Chinese Medicine called our money manager to consult on the sample processing method. After some answers from the money manager, the question of Hu was successfully lifted. The following is the sample processing method compiled by our technical department. :
1.
How to treat serum samples?
Whole blood specimens are placed at room temperature
2
Hour or
4
After °C overnight
1000
×
g
Centrifugation
20
minute. Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
2.
How is the plasma sample treated?
Recommended choice
EDTA
Or sodium citrate as an anticoagulant, mixed
10-20
Minutes later, after
1000
×
g
Centrifugation
15
Minutes, carefully collect the supernatant. If a precipitate forms during storage, it should be centrifuged again.
3.
How to treat cell supernatant samples?
When detecting secreted components, collect them in a sterile tube. Centrifugation
20
Minutes or so
1000
×
g
). Collect the supernatant carefully.
4. How to treat cell lysate samples?
When detecting intracellular components, use
PBS
(
PH7.2-7.4
Dilute the cell suspension and reach the cell concentration
100
Ten thousand
/ml
about. By repeated freezing and thawing or sonication, the cells are destroyed and the intracellular components are released. Centrifugation
10
Minutes or so
5000
×
g
). Collect the supernatant carefully. If a precipitate forms during storage, it should be centrifuged again.
5.
How is the organization sample processed?
Pre-cooled PSB (0.01M, pH=7.4)
Rinse the tissue to remove residual blood (cleaved red blood cells in the homogenate will affect the measurement) and weigh the tissue after weighing. The shredded tissue and the corresponding volume
PBS
(Generally press
1:9
Weight to volume ratio, such as
1g
Tissue sample corresponding
9mL
of
PBS
The specific volume can be adjusted according to the needs of the experiment and recorded. Recommended in
PBS
The protease inhibitor was added to the glass homogenizer and thoroughly ground on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly thawed. Finally, the homogenate is
5000
×
g
Centrifugation
5~10
Minutes, take the supernatant test.
Are you clear about the sample processing method? If you have any questions, please call us. Shanghai Hengyuan Biotechnology Co., Ltd. has a complete experimental technology development platform, mature antigen and antibody research and development system, proficient in various enzyme-linked technologies, combined with the company's research and development team, can effectively ensure the company's products with strong stability and high Accuracy, while at the same time having the most competitive price. At present, the company can provide biochemical reagents, molecular biological reagents and kits, various antibodies and immunological kits, experimental consumables and many other tens of thousands of products, including molecular biology, cell biology, immunology and other life sciences. each field.
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