White blood cell activation adhesion factor (ALCAM) detection kit overview - Database & Sql Blog Articles

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Leukocyte Activated Adhesion Factor (ALCAM) Assay Kit
ELISA Kit for Activated Leukocyte Cell Adhesion Molecule (ALCAM)

For in vitro studies only, not for clinical diagnosis!

• Adhesion molecule
  • Applicable species

    Homo sapiens (Human, person)

    • experimental method

      Double-anti-sandwich method

    • specification

      48T, 96T , 96T×5, 96T×10, 96T×100

      • examination range

        0.156-10ng/mL

      • Sensitivity

        The minimum detectable dose is less than or equal to 0.059ng/mL.

        • download

          English manual

          User manual in Chinese

          
          Product packaging

          

          Experimental result chart

          

          Standard curve

          

          Passed ISO 9001, ISO 13485 quality system certification

          Specificity

          This kit is used to detect leukocyte-activated adhesion factor (ALCAM) and has no significant cross-reactivity with other similar substances.
          Due to technical and sample source limitations, cross-reactivity testing of all related or similar substances is not possible, so the kit may cross-react with other substances that are not detected.

          Recovery rate

          A certain amount of leukocyte-activated adhesion factor (ALCAM) (spiked sample) was added to the fixed-value serum and plasma samples, and the average was repeated and the ratio was calculated as the ratio of the measured value to the theoretical value.

          Sample recovery range (%) Average recovery (%) serum(n=5) 92-101 95 EDTA plasma(n=5) 89-104 98 heparin plasma(n=5) 86-104 95

          Precision

          The precision is expressed by the coefficient of variation CV of the measured value of the sample. CV(%) = SD/mean×100

          
          Batch internal difference: Take the same batch of kits for quantitative detection of low, medium and high value fixed samples. Each sample is continuously measured 20 times, and the average value and SD value of different concentration samples are calculated separately.
          Batch-to-batch difference: Select three different batches of kits to quantitatively determine low, medium and high-valued samples, and repeat each test with the same kit for 8 times to calculate the average and SD of different concentrations of samples. value.
          Batch difference: CV<10%
          
          Inter-batch difference: CV<12%
          
          

          Linear

          Add appropriate amount of leukocyte-activated adhesion factor (ALCAM) to the serum and plasma samples, and dilute them to 1:2, 1:4, 1:8, 1:16 samples. The linear range is after dilution. The ratio of the measured value of the leukocyte-activated adhesion factor (ALCAM) content to the theoretical value in the sample.

          Sample 1:2 1:4 1:8 1:16 serum(n=5) 91-98% 82-96% 78-104% 83-92% EDTA plasma(n=5) 93-105% 89-101% 88-97% 80-93% heparin plasma (n=5) 90-103% 96-105% 97-105% 96-105%

          stability

          It has been determined that the kit is stored at the recommended temperature during the period of validity, and its activity reduction rate is less than 5%.
          In order to reduce the influence of external factors on the detection values ​​before and after the damage of the kit, the environmental conditions of the laboratory should be kept as consistent as possible, especially the temperature, humidity and incubation conditions in the laboratory. Secondly, the same experimenter can operate to reduce human error.

          Kit content

          Reagent name quantity reagent name number 96-well plate (pre-coated) 1 96-well plate coating 4 standard 2 standard dilution 1 × 20mL detection solution A 1 × 120μL detection dilution A 1 × 12mL detection solution B 1 × 120μL Detection dilution B 1×12mL TMB substrate 1×9mL Stop solution 1×6mL Concentrated washing solution (30 × concentrate) 1×20mL Instruction 1

          experiment process

          1. Preparation of standards, reagents and samples before the experiment;
          2. Add 100 μL of sample (standard and sample) and incubate for 2 hours at 37 °C;
          3. Aspirate, add 100 μL of detection solution, and incubate for 1 hour at 37 °C;
          4. Wash the plate 3 times;
          5. Add 100 μL of test solution B and incubate at 37 ° C for 30 minutes;
          6. Wash the plate 5 times;
          7. Add 90 μL of TMB substrate and incubate at 37 ° C for 15-25 minutes;
          8. Add 50 μL of stop solution and read immediately at 450 nm.
          

          
          

          Experimental principle

          The leukocyte-activated adhesion factor (ALCAM) antibody is coated in a 96-well microplate to prepare a solid phase carrier, and a standard or a sample is added to the microwell, wherein the leukocyte-activated adhesion factor (ALCAM) is linked to the solid phase. The antibody on the carrier is bound, then biotinylated leukocyte-activated adhesion factor (ALCAM) antibody is added, the unbound biotinylated antibody is washed, HRP-labeled avidin is added, and the TMB substrate is added again after thorough washing. color. TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with the white blood cell activation adhesion factor (ALCAM) in the sample. The absorbance (OD value) was measured at a wavelength of 450 nm using a microplate reader to calculate the sample concentration.

          related products

          No. Applicable species: Homo sapiens (Human, human) Application of SEA002Hu Leukocyte Activated Adhesion Factor (ALCAM) Assay Kit (Enzyme-Linked Immunosorbent Assay) Enzyme-linked immunosorbent assay SCA002Hu Leukocyte Activated Adhesion Factor (ALCAM) Assay Kit (Chemiluminescence) Immunoassay) Chemiluminescent immunoassay PAA002Hu01 Leukocyte Activated Adhesion Factor (ALCAM) Polyclonal Antibody WB, ICC, IHC-P, IHC-F, ELISA MAA002Hu22 Leukocyte Activation Adhesion Factor (ALCAM) Monoclonal Antibody WB, ICC, IHC-P, IHC -F, ELISA RPA002Hu01 Leukocyte Activated Adhesion Factor (ALCAM) Recombinant Protein SDS-PAGE; WB; ELISA; IP. LAA002Hu71 Leukocyte Activated Adhesion Factor (ALCAM) Biotinylated Antibody IHC; WB; ELISA LAA002Hu81 Leukocyte Activated Adhesion Factor (ALCAM) Fluorescein thiocyanate-labeled antibody IHC; ICC; IF; WB The main problems of biochemical determination are the following aspects: reviewing the literature selection method, preparing reagents, pre-experiment to determine whether the method is suitable for your research materials, and repeatedly adjusting to optimize the measurement system and measurement steps. For graduate students with insufficient research experience and measurement training, the above-mentioned troubles often lead to repeated failures in measurement, resulting in a large waste of time, samples and reagents, and inability to obtain reliable data in time.
          
          The use of a kit is equivalent to outsourcing the above-mentioned annoyance work to a professional company. Compared with other companies, Jinma Bio has won the trust and support of users through a practical series of kits, practical comprehensive services and affordable prices. Therefore, the biochemical assay is the preferred kit.
          
          4 reasons you choose:
          1. An agent in mainland China, a formal and legal purchase channel, and a large number of agency brands;
          2, the whole process of cold chain transportation to ensure product quality;
          3. Efficient pre-sales and after-sales service to provide comprehensive product consultation;
          4. Professional technical support team to ensure the correct use of the product.