Nine reagents in ELISA kit operation - Database & Sql Blog Articles

It is very important that the preparation of the nine reagents before the experiment is very important. The operator needs to prepare the required materials before the experiment starts. In the ELISA kit operation, materials such as distilled water, sampler, oscillator, magnetic stirrer, etc. need to be prepared. Today, Shanghai Hengyuan will tell you about the nine reagents that are indispensable:
Diluent
2. ABTS use fluid
3. Antigen, antibody and enzyme-labeled antibodies
4. Normal human serum and positive control serum
5. Stop solution (2M H2SO4)
6. Substrate buffer (pH 5.0 phosphate citrate)
7. Coating buffer (pH 9.6 0.05M carbonate buffer)
8. Wash Buffer (pH 7.4 PBS): 0.15M
9. TMB (tetramethylbenzidine) use solution
ELISA is an immunoassay that uses immunological techniques to determine specimens. Specimens that can be used as ELISA assays are widely available, and body fluids, secretions, and excretions can be used as specimens to determine an antibody or antigen component. Some specimens can be directly tested by ELISA kits, while others require pretreatment.